Secondary antibodies utilized were FITC-conjugated goat anti-mouse, FITC-conjugated donkey anti-rabbit (pre-cleared), and rhodamine-conjugated goat anti-rabbit (Jackson ImmunoResearch Laboratories Inc) used at 1:100. A and B) Duplicate membranes were probed with the antibodies indicated above and developed with Pierce Pico substrate (or the more sensitive Femto substrate where indicated). The arrow shows the poor 29 kDa […]