Movies (25 frames, each 0.2?s) were collected at a defocus range of ?2.8 to ?0.8?m in single-electron counting mode with SerialEM37 Sigma-1 receptor antagonist 2 at a calibrated magnification of 37,037, resulting in a pixel size of 1 1.35?? per pixel. Data collection and refinement statistics are summarized in Supplementary Table?1. Image control and three-dimensional […]