The membranes were blocked with 5% skim dairy in TBS-T (containing 0.2% Tween-20) at RT with gentle shaking for 60?a few minutes, accompanied by incubation with primary antibodies (1:1,000 dilution in 5% skim dairy) for 1C1.5?hours in RT or overnight (4C) with soft shaking. myotubes induced by lovastatin, another muscles cell loss of life inducer, […]