A cDNA fragment containing sequences encoding proteins 885-1301 from the mouse TEX14 proteins was subcloned into pET23b (Novagen), His-tagged TEX14 was stated in BL21 (DE3) pLysS cells, and polyclonal antibodies were raised in guinea pigs (Cocalico Biologicals, Reamstown, PA). For a thorough description of the techniques and components find em Supporting Materials and Methods /em […]