Chromatography was performed in PBS, pH 7.4, at 0.4 ml/min in the KTA-Purifier 10 (GE Healthcare). with the 180-kDa molecule IgE recognized a large positively charged binding surface that includes a flexible loop, which is unique to the IPSE/-1 crystallin collapse. Mutational analysis of amino acids in the binding interface showed that residues contributing to […]