RNA was prepared from each sort-purified B cell subset and reverse transcribed. in C57BL/6 B cells. Peritoneal washout cells and spleen cells were obtained from 3-month-old C57BL/6J mice, immunofluorescently stained, and sorted for peritoneal B-1a (B220loCD5+), CD25+ B-1a (B220loCD5+CD25+), CD25? B-1a (B220loCD5+CD25?), splenic B2 (B220+CD5?CD23+), and GC (B220+/GL-7+/PNAhigh) cells, as shown in Physique ?Physique1.1. RNA […]