Dilutions of 3-PBA in PBS was blended with a 1/2500 dilution from the phage-VHH in PBS and 100 l from the mix was added into wells. assay was extremely particular to 3-PBA and its own 4-hydroxylated derivative, 4-hydroxy 3-PBA (150% combination reactivity) with negligible combination reactivity with various other examined structural analogs as well as […]