The bright red immunofluorescence-staining pattern in the Zpl 2-1-PrP cells revealed that this prion protein was distributed on the surface of the cells, whereas no immunoreactivity was detected in the Zpl 2-1-vector cells. Zpl 2C1 (A) and of Zpl 2-1-vector and Zpl 2-1-PrP (B) cells treated with the indicated concentrations of Mn2+. Transmission light microscopy […]