Briefly, 10 l of CCK-8 remedy was added to each well of a 96-well plate and incubated at 37C for 4 h. YVAD-cmk, suggesting that E2-induced cell death was associated with caspase 1-dependent pyroptosis. Second, the key role of the NLRP3 inflammasome in autophagy of HCC cells was assessed by E2-induced activation of the NLRP3 […]