Protein focus in the supernatant (STAg) was dependant on Lowry technique [18], as well as the antigen was stored at -20?C until make use of. Phenolic Glycolipid-1 (PGL-1) ELISA IgG and IgM anti-PGL-1 antibodies were detected by enzyme-linked immunosorbent assay (ELISA) seeing that previously described by Bazan-Furini [19], with some adjustments. IgM anti-PGL-1 antibodies had […]